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[DOI] [PMC free article] [PubMed] [Google Scholar] 105.Yayama K, Hiyoshi H, Imazu D, Okamoto H

Some neurons appear to be both cholinergic and nitrergic, and there is a population of cells that do not express the rate-limiting enzymes for the production of either ACh (cholineacetyltransferase, ChAT) or nitric oxide (nitric oxide synthase, NOS)

Alexa Fluor conjugated secondary antibodies were used to fluorescently label the antigens of interest and following dehydration in a series of increasing concentrations of methanol and cleared using dibenzyl ether, the membrane-bound slices were placed onto a glass microscope slide and imaged using a Zeiss LSM-800 inverted confocal microscope

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