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The assay was performed in a plate 96 wells Sterilin (Fisher Scientific, UK) where 10 L of homogenate was transferred to each well containing 180 L of 0.1 M buffer sodium phosphate pH 8.0, 1 mM EDTA, 10 L of 10 mM 5,5-dithiobis (2-nitrobenzoic acid) (DTNB)
Higher serum free testosterone is associated with better cognitive function in older men, while total testosterone is not
Mandatory statement: A dietary supplement is not a substitute for a varied, balanced diet and a healthy lifestyle
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