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Unraveling the crucial roles of FoxP3+ regulatory T cells in vascularized composite allograft tolerance induction and maintenance

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DNA templates for in vitro transcription of sgRNA were generated by annealing a scaffold oligonucleotide sequence: AAAGCACCGACTCGGTGCCACTTTTTCAAGTTGATAACGGACTAGCCTTATTTTAACTTGCTATTTCTAGCTCTAAAAC, with gene-specific oligonucleotides containing the SP6 promoter sequence (GCGATTTAGGTGACACTATA), a 20-base target sequence (see Table S1), and a complementary sequence to the scaffold oligo (GTTTTAGAGCTAGAAATAG)