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Woods KA, Camacho-Hubner C, Savage MO, Clark AJ

The bait protein, along with any bound prey proteins, is then captured using techniques like affinity beads, which could be coated with the antibody or ligand

This creates positive pressure inside the vial, making it much easier to draw water out

Heat production from uncoupling electron transport would be another- this is thought to lead to death before ATP depletion