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For those without contraindications of vitamin B12, the potential benefits of vitamin B12 supplementation are often deemed to outweigh the associated risks

For miRNA analysis, approximately 100 ng of total RNA was reverse-transcribed using M-MLV reverse transcriptase (Promega, M1705) with specific stemloop primers for miRNAs and a reverse primer for U6 (Table 1)
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The tomato HD-zip I transcription factor SlHZ24 modulates ascorbate accumulation through positive regulation of the d-mannose/L-galactose pathway