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Notwithstanding the remarkable effects of Zn in cell biology, the wide range of Zn effects may be largely dependent on Zn concentration and cell type and may even differ considerably under normal and pathological conditions
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Recently, high-throughput proteomics studies have used GST fusion proteins for directional immobilization in protein microarrays (6) and surface plasmon resonance (7)
However the main disadvantages of this host are related to regulatory demands and production time (Wilken and Nikolov, 2012)
