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Heart nuclei were isolated using a lysis buffer consisting of 0.25M sucrose, 10mM Tris-Hcl pH 7.5, 25mM KCl, 5mM MgCl2, 45uM Actinomycin D, supplemented with 1X protease inhibitor (G6521, Promega), 0.4U/uL RNasin Ribonuclease Inhibitor (N2515, Promega), 0.2U/ul SuperaseIn (AM2694, ThermoFisher)

ALT levels improved (76 vs 50 U/L, p = 0.001)

The medication appears to reduce both physiological hunger and the reward value of food, making it easier to distinguish physical hunger from emotional cravings

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